Journal: Neoplasia (New York, N.Y.)

Article Title: Nephron Progenitor But Not Stromal Progenitor Cells Give Rise to Wilms Tumors in Mouse Models with β-Catenin Activation or Wt1 Ablation and Igf2 Upregulation 1

doi: 10.1016/j.neo.2015.12.001

Figure Lengend Snippet: Summary of Mouse Mutant Cohorts and TM Injection Conditions

Article Snippet: Antibodies used were: WT1 (sc-192, Santa Cruz Biotechnology), Ki67 (ab15580, Abcam), pHH3 (06-570, Upstate Biotechnology), β-catenin (610154, BD Biosciences), Dlk1 (sc-8624, Santa Cruz Biotechnology), Cited1 (9219, Fisher Scientific), Six2 (11562-1-AP, Proteintech), Pax2 (PRB-276P, Covance), NCAM (C9672, Sigma), E-cadherin (3195, Cell Signaling Technology), K-cadherin (ab79005, Abcam), Vimentin (V2258, Sigma), Collagen IV (AB756P, Chemicon), Cyclin D1 (sc-753, Santa Cruz Biotechnology), and C-myc (9E10, Santa Cruz Biotechnology).

Techniques: Mutagenesis, Injection

Journal: Neoplasia (New York, N.Y.)

Article Title: Nephron Progenitor But Not Stromal Progenitor Cells Give Rise to Wilms Tumors in Mouse Models with β-Catenin Activation or Wt1 Ablation and Igf2 Upregulation 1

doi: 10.1016/j.neo.2015.12.001

Figure Lengend Snippet: Development of WT from nephron progenitors. (A) Kaplan-Meier tumor-free survival analysis showing incidence of WT in Six2 GCE , Cited1 Cre , and Foxd1 GCE mutant mice with Wt1 ablation/haploinsufficiency and β-catenin stabilization ( S/C/F-Wt1 −/fl -β-cat S and S/C/F-Wt1 +/fl -β-cat S ) and Wt1 ablation and Igf2 upregulation ( S/C/F-Wt1 −/fl -Igf2 ) in comparison to control mice. (B) PCR analysis of DNA from mutant mice showing Ctnnb1 wild-type or exon3 floxed allele and exon3 deleted allele ( Ctnnb1 + or Ctnnb1 ex3(fl) and Ctnnb1 ex3Δ ) and Wt1 floxed or null alleles (Wt1 fl or Wt1 Δ ).

Article Snippet: Antibodies used were: WT1 (sc-192, Santa Cruz Biotechnology), Ki67 (ab15580, Abcam), pHH3 (06-570, Upstate Biotechnology), β-catenin (610154, BD Biosciences), Dlk1 (sc-8624, Santa Cruz Biotechnology), Cited1 (9219, Fisher Scientific), Six2 (11562-1-AP, Proteintech), Pax2 (PRB-276P, Covance), NCAM (C9672, Sigma), E-cadherin (3195, Cell Signaling Technology), K-cadherin (ab79005, Abcam), Vimentin (V2258, Sigma), Collagen IV (AB756P, Chemicon), Cyclin D1 (sc-753, Santa Cruz Biotechnology), and C-myc (9E10, Santa Cruz Biotechnology).

Techniques: Mutagenesis, Comparison, Control

Journal: Neoplasia (New York, N.Y.)

Article Title: Nephron Progenitor But Not Stromal Progenitor Cells Give Rise to Wilms Tumors in Mouse Models with β-Catenin Activation or Wt1 Ablation and Igf2 Upregulation 1

doi: 10.1016/j.neo.2015.12.001

Figure Lengend Snippet: Histological analysis of WTs from Six2 GCE and Cited1 Cre mice. (A) Gross appearance of tumors from Six2 GCE mice (a) and Cited1 Cre mice (b and c) of the indicated genotypes. (B) H&E staining of kidney tumor sections of S-Wt1-β-cat S , C-Wt1-β-cat S , and C-Wt1-Igf2 mice. Scale bar: 100 μm.

Article Snippet: Antibodies used were: WT1 (sc-192, Santa Cruz Biotechnology), Ki67 (ab15580, Abcam), pHH3 (06-570, Upstate Biotechnology), β-catenin (610154, BD Biosciences), Dlk1 (sc-8624, Santa Cruz Biotechnology), Cited1 (9219, Fisher Scientific), Six2 (11562-1-AP, Proteintech), Pax2 (PRB-276P, Covance), NCAM (C9672, Sigma), E-cadherin (3195, Cell Signaling Technology), K-cadherin (ab79005, Abcam), Vimentin (V2258, Sigma), Collagen IV (AB756P, Chemicon), Cyclin D1 (sc-753, Santa Cruz Biotechnology), and C-myc (9E10, Santa Cruz Biotechnology).

Techniques: Staining

Journal: Neoplasia (New York, N.Y.)

Article Title: Nephron Progenitor But Not Stromal Progenitor Cells Give Rise to Wilms Tumors in Mouse Models with β-Catenin Activation or Wt1 Ablation and Igf2 Upregulation 1

doi: 10.1016/j.neo.2015.12.001

Figure Lengend Snippet: Activity of Wnt/β-catenin signaling in mouse WTs. (A) IHC staining of β-catenin and C-myc for sections from control kidneys and tumors from S-Wt1-β-cat S , C-Wt1-β-cat S , and C-Wt1-Igf2 mutants. Scale bar: 100 μm. (B) qPCR analysis of Wnt/β-catenin canonical effectors Axin2 , CyclinD1 , and C-myc and signaling inhibitors Wif1 and Dkk2 in littermate kidneys and tumors from S-Wt1-β-cat S , C-Wt1-β-cat S , and C-Wt1-Igf2 mutants. The x -axis labels indicate wild-type (Wt1 +/fl ) in littermate kidneys and the presence of progenitor-specific Cre alleles ( Six2 GCE or Cited1 Cre ) or Ubiquitous-Cre (U) and the presence of genetic alterations of β-cat S , Wt1 ablation ( Wt1 −/∆ ) or Igf2 upregulation ( H19 +/− m ) in mouse tumors.

Article Snippet: Antibodies used were: WT1 (sc-192, Santa Cruz Biotechnology), Ki67 (ab15580, Abcam), pHH3 (06-570, Upstate Biotechnology), β-catenin (610154, BD Biosciences), Dlk1 (sc-8624, Santa Cruz Biotechnology), Cited1 (9219, Fisher Scientific), Six2 (11562-1-AP, Proteintech), Pax2 (PRB-276P, Covance), NCAM (C9672, Sigma), E-cadherin (3195, Cell Signaling Technology), K-cadherin (ab79005, Abcam), Vimentin (V2258, Sigma), Collagen IV (AB756P, Chemicon), Cyclin D1 (sc-753, Santa Cruz Biotechnology), and C-myc (9E10, Santa Cruz Biotechnology).

Techniques: Activity Assay, Immunohistochemistry, Control

Journal: Neoplasia (New York, N.Y.)

Article Title: Nephron Progenitor But Not Stromal Progenitor Cells Give Rise to Wilms Tumors in Mouse Models with β-Catenin Activation or Wt1 Ablation and Igf2 Upregulation 1

doi: 10.1016/j.neo.2015.12.001

Figure Lengend Snippet: Expression of differentiation markers in WTs of Six2 GCE and Cited1 Cre mice. qPCR analysis of early metanephric mesenchyme markers ( Eya1 , Osr1 , Pax2 , Hoxa11 , and Hmga2 ) and renal vesicle markers ( Wnt4 and Jag1 ) in littermate kidneys and tumors from S-Wt1-β-cat S , C-Wt1-β-cat S , and C-Wt1-Igf2 mutants. The x -axis labels indicate wild-type (Wt1 +/fl ) in littermate kidneys and the presence of progenitor-specific Cre alleles ( Six2 GCE or Cited1 Cre ) or Ubiquitous-Cre (U) and the presence of genetic alterations of β-cat S , Wt1 ablation ( Wt1 −/∆ ) or Igf2 upregulation ( H19 +/− m ) in mouse tumors.

Article Snippet: Antibodies used were: WT1 (sc-192, Santa Cruz Biotechnology), Ki67 (ab15580, Abcam), pHH3 (06-570, Upstate Biotechnology), β-catenin (610154, BD Biosciences), Dlk1 (sc-8624, Santa Cruz Biotechnology), Cited1 (9219, Fisher Scientific), Six2 (11562-1-AP, Proteintech), Pax2 (PRB-276P, Covance), NCAM (C9672, Sigma), E-cadherin (3195, Cell Signaling Technology), K-cadherin (ab79005, Abcam), Vimentin (V2258, Sigma), Collagen IV (AB756P, Chemicon), Cyclin D1 (sc-753, Santa Cruz Biotechnology), and C-myc (9E10, Santa Cruz Biotechnology).

Techniques: Expressing

Journal: Molecular Metabolism

Article Title: P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response

doi: 10.1016/j.molmet.2020.101089

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: Wilms Tumor-1 (WT1) , Boster Biological Technology , #M00199-1 , Rabbit , 1 μg/ml (IHC).

Techniques:

Journal: Molecular Metabolism

Article Title: P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response

doi: 10.1016/j.molmet.2020.101089

Figure Lengend Snippet: P2Y2R deficiency protects against podocyte loss and glomerular injury in DN. (A) Relative mRNA levels of Nphs1 (nephrin) and Nphs2 (podocin) were determined by real-time PCR analysis (n = 3–5). (B) Localisation of WT1, a podocyte marker, was analysed by immunohistochemistry, and representative images are shown. The number of stained podocytes per glomeruli was counted by using ImageJ software (n = 3). (C) Kidney sections were stained with PAS staining and glomerular morphological changes were scored as described in the method section (n = 3). Data are presented as mean ± SEM. One-way ANOVA was used for statistical analysis followed by Bonferroni's multiple comparisons test. ∗∗∗p < 0.001 vs WT control mice; and ### p < 0.001 vs WT DN mice. Scale bar, 50 μm.

Article Snippet: Wilms Tumor-1 (WT1) , Boster Biological Technology , #M00199-1 , Rabbit , 1 μg/ml (IHC).

Techniques: Real-time Polymerase Chain Reaction, Marker, Immunohistochemistry, Staining, Software, Control

Journal: Scientific Reports

Article Title: Identification of m6A methyltransferase-related WTAP and ZC3H13 predicts immune infiltrates in glioblastoma

doi: 10.1038/s41598-025-88671-4

Figure Lengend Snippet: Relative expression of genes expression in GBM tissues. Expression levels of WTAP and ZC3H13 genes were compared with those of the control group on GBM tissues, 10 GBM and adjacent tissues, respectively ( A ). The expression levels of siRNA WTAP and overexpression ZC3H13 genes ( B and C ). The expression levels of CD27, CD70, CD80, CD86, ICOS, CTLA4, and LAG3 in siRNA WTAP and overexpression ZC3H13 were demonstrated in Fig. 9D and F, respectively. The result were determined by qRT-PCR. The data are mean ± standard deviation of six replicate experiments. * P < 0.05, **P < 0.01 and ***P < 0.001 compared with the corresponding control values.

Article Snippet: After blocking with 5% nonfat milk, the membranes were immunoblotted with the primary antibodies: WTAP (A04296-2, Boster Biotech, Wuhan, China), CD27 (A01148-2, Boster Biotech), CD70 (A02853-2, Boster Biotech), CD80 (A00196-3, Boster Biotech), CD86(BM4121, Boster Biotech), ICOS (A00291-2, Boster Biotech), CTLA4 (A00020-1, Boster Biotech), LAG3 (M02869-2, Boster Biotech), Beta-actin (BM3873, Boster Biotech).

Techniques: Expressing, Control, Over Expression, Quantitative RT-PCR, Standard Deviation

Journal: International Journal of Biological Sciences

Article Title: APT1-derived depalmitoylation of CD36 alleviates diabetes-induced lipotoxicity in podocytes

doi: 10.7150/ijbs.109220

Figure Lengend Snippet: Construction of db/db model to evaluate changes in renal pathology lipid metabolism. Two groups of twenty-week-old mice were analyzed: db/m (n=6) and db/db (n=6). A-B. Representative PAS staining of glomeruli in each group, and the semiquantitative analysis of mesangial area ratio. (**<0.01, scale bar: 50 μm). C-G. Biochemical examination: (C) UACR, (D) serum creatinine, (E) blood glucose, (F) serum cholesterol, (G) serum triglycerides. (**<0.01). H. Representative transmission electron microscopy (TEM) images of podocyte foot processes and glomerular basement membrane (GBM) in each group. (**<0.01, scale bar: 10 μm). I-J. Representative immunofluorescence double staining of BODIPY and WT1 in the glomeruli of db/m and db/db mice, and semiquantitative analysis. (**<0.01, scale bar:50μm). K-L. Oil Red O staining of lipid droplets (LDs) in the glomeruli of db/m and db/db mice, and semiquantitative analysis. (**<0.01, scale bar: 40 μm).

Article Snippet: Frozen kidney sections were fixed, blocked, and then stained with WT1 antibody (BM4216, Boster, China) for 1 h at 37°C, subsequently incubated in the presence of secondary antibody and BODIPY dye, and finally covered with DAPI.

Techniques: Staining, Transmission Assay, Electron Microscopy, Membrane, Immunofluorescence, Double Staining